Equipment

The Tecnai G2 Spirit BioTWIN Transmission Electron Microscope is a general-purpose instrument specifically suited for low contrast samples. The microscope is equipped with an AMT Nanosprint 43-MKII camera.
Sign-Up
Tecnai TEM Operation Manual

The JEOL 1200EX is a conventional transmission electron microscope equipped with an AMT 2k CCD camera.
Sign-Up
JEOL TEM Operation Manual

The JEM-120i transmission electron microscope can be operated at accelerating voltages ranging from 80 kV to 120 kV. It features an enhanced TEM control system and fully automated apertures, making it easy to use even for less experienced operators. The microscope is equipped with an AMT NanoSprint 15-MKII camera, enabling high-resolution digital imaging for various analytical applications.
Sign Up

The Hitachi S-4700 FE-SEM is a cold field emission high resolution scanning electron microscope. With an accelerating voltage ranging from 0.5 to 30kV the FE-SEM has a magnification range between 30X and 500,000X with spatial resolution of up to 1.5 nm.
Sign-Up
SEM Operation Manual

Microtome for ultrathin plastic sectioning (30nm-2um).
•Email to get trained on this instrument.
•If you're already trained, Sign Up after communicating with staff about availability.
Thick Sectioning Manual
Thin Sectioning Manual

Ultrathin frozen sections (60-90nm) are routinely used for immunogold localization (Tokayasu method).
Sign Up

Glow Discharge Unit applies a negative charge which makes things hydrophilic.

Dries samples at the critical point to preserve structure for SEM samples.
CPD Operation Manual

Leica EM ACE600 is a high vacuum film deposition system designed to produce very thin, fine-grained and conductive metal and carbon coatings for highest resolution analysis, as required for your FE-SEM and TEM applications.
Sign-Up
Carbon Coating Manual
Sputter Coating Manual

For improved fine structure preservation samples can be frozen under high pressure (2000 bar), freeze substituted at low temperature, and embedded in resin. HPF is especially important for some difficult-to-fix samples such as yeast, drosophila, and C. elegans. The technique also retains antigenicity of the sample well, making it useful for immuno EM. Sign-Up

Automatic Freeze-Substitution System.
Freeze Substitution Manual

For easy trimming of plastic blocks.