#  Negative Staining 

 



##  Negative Staining 

 

 

       ![Negative Stained Bacteria Phage](/sites/g/files/omnuum4896/files/styles/hwp_21_9__1920x825/public/electron-microscopy/files/negative_stained_phage.jpg?h=6cf9f2c6&itok=BJPiOTXJ) 

 

 



 

 



 

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5µl of the sample is adsorbed for 1 minute to a carbon coated grid that has been made hydrophilic by a 20 second exposure to a glow discharge (25mA). Excess liquid is removed with a filterpaper (Whatman #1), the grid is then floated briefly on a drop of water (to wash away phosphate or salt), blotted again on a filer paper and stained on a drop of 0.75% uranyl formate (EMS catalog # 22451) or 1% Uranyl Acetate (EMS catalog # 22400) for 20-30 seconds. After removing the excess stain with a filter paper the grids can be examined in a Transmission electron microscope.



 

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 Attachments- [  picture\_as\_pdf  Negative Staining Procedure.pdf ](/sites/g/files/omnuum4896/files/2024-11/Negative%20Staining%20Procedure_0.pdf)
 
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